Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceMAPWQCAPCS AEKLALCPPV SASCSEVTRS AGCGCCPMCA LPLGAACGVA TARCARGLSC RALPGEQQPL HALTRGQGAC VQESDASAPH AAEAGSPESP ESTEITEEEL LDNFHLMAPS EEDHSILWDA ISTYDGSKAL HVTNIKKWKE PCRIELYRVV ESLAKAQETS GEEISKFYLP NCNKNGFYHS RQCETSMDGE AGLCWCVYPW NGKRIPGSPE IRGDPNCQIY FNVQN

Specifications

Our Abpromise guarantee covers the use of ab50216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityBiological Activity : The ED50, as determined by its ability to inhibit IGF-I induced proliferation of MCF-7, is < 0.5 µg/ml in the presence of 6 ng/ml of human IGF-I.
  • Applications

    Western blot

    SDS-PAGE

    Functional Studies

  • Endotoxin level< 0.100 Eu/µg
  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionReconsitute to 1mg/ml with sterile water.

General Info

  • Alternative names
    • AFBP
    • Alpha pregnancy associated endometrial globulin
    • Amniotic fluid binding protein
    • Binding protein 25
    • Binding protein 26
    • Binding protein 28
    • Growth hormone independent binding protein
    • hIGFBP 1
    • hIGFBP1
    • IBP 1
    • IBP-1
    • IBP1
    • IBP1_HUMAN
    • IGF binding protein 1
    • IGF BP25
    • IGF-binding protein 1
    • IGFBP 1
    • IGFBP-1
    • IGFBP1
    • Insulin like growth factor binding protein 1
    • Insulin-like growth factor-binding protein 1
    • Placental protein 12
    • PP 12
    • PP12
    see all
  • FunctionIGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.
  • Sequence similaritiesContains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • Post-translational
    modifications
    Phosphorylated; probably by casein kinase II. Alters the affinity of the protein for IGFs.
  • Cellular localizationSecreted.
  • Information by UniProt

Recombinant human IGFBP1 protein images

  • Anti-IGFBP1 antibody [33627.11] (ab94985) at 1 µg/ml + Recombinant human IGFBP1 protein (ab50216) at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

References for Recombinant human IGFBP1 protein (ab50216)

ab50216 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"