Recombinant human IGFBP2 protein (ab63223)

Overview

Description

  • NatureRecombinant
  • SourceInsect cells
  • Amino Acid Sequence
    • AccessionP18065
    • SpeciesHuman
    • SequenceEVLFRCPPCT PERLAACGPP PVAPPAAVAA VAGGARMPCA ELVREPGCGC CSVCARLEGE ACQVYTPRCG QGLRCYPHPG SELPLQALVM GEGTCEKRRD AEYGASPEQV ADNGDDHSEG GLVENHVDST MNMLGGGGSA GRKPLKSGMK ELAVFREKVT EQHRQMGKGG KHHLGLEEPK KLRPPPARTP CQQELDQVLE RISTMRLPDE RGPLEHLYSL HIPNCDKHGL YNLKQCKMSL NGQRGECWCV NPNTGKLIQG APTIRGDPEC HLFYNEQQEA RGVHTQRMQ
    • Molecular weight32 kDa
    • Amino acids37 to 325
    • Additional sequence informationThe sequence is the full length mature form minus the signal peptide.

Specifications

Our Abpromise guarantee covers the use of ab63223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Biological Activity : Determined by its ability to inhibit IGF-I induced proliferation of FDC-P1 cells.

  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Endotoxin level< 1.000 Eu/µg
  • Purity> 98 % SDS-PAGE.
    Greater than 98% by SDS-PAGE gel and HPLC analyses. Endotoxin level is < 0.1 ng/µg of protein (<1EU/µg).
  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • BP 2
    • BP2
    • IBP 2
    • IBP-2
    • IBP2
    • IBP2_HUMAN
    • IGF binding protein 2
    • IGF BP53
    • IGF-binding protein 2
    • IGFBP 2
    • IGFBP-2
    • IGFBP2
    • IGFBP53
    • Insulin like growth factor binding protein 2
    • Insulin like growth factor binding protein 2 36kDa
    • Insulin like growth factor-binding protein 2 precursor
    • Insulin-like growth factor-binding protein 2
    see all
  • FunctionInhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
  • Sequence similaritiesContains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • DomainThe C-terminus is required for IGF-binding and growth inhibition.
  • Post-translational
    modifications
    O-glycosylated.
  • Cellular localizationSecreted.
  • Information by UniProt

Recombinant human IGFBP2 protein images

  • Anti-IGFBP2 antibody (ab91404) at 1/500 dilution + Recombinant human IGFBP2 protein (ab63223) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

References for Recombinant human IGFBP2 protein (ab63223)

ab63223 has not yet been referenced specifically in any publications.

Product Wall

A serum-free media is used for culturing. However, please note that the virus stock contains serum and the final concentration of the serum is 0.1% FBS in the production.

This product is provided is free of any carrier proteins.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"