The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityThe ED50 range is 0.01-0.09µg/ml as measured by its ability to inhibit the biological activity of IGFII (20ng/ml) on MCF7 human breast adenocarcinoma cells.
% SDS-PAGE. ab125753 is purified by proprietary chromatographic techniques.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C. Store under Desiccating conditions.
Constituent: 99% PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionIt is recommended to reconstitute ab125753 in sterile ultra pure water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Upon reconstitution ab125753 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
IGF binding protein 4
IGF-binding protein 4
Insulin Like Growth Factor Binding Protein 4
Insulin-like growth factor-binding protein 4
FunctionIGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.