Recombinant human IGFBP4 protein (ab125753)

Overview

Description

  • NatureRecombinant
  • SourceHEK 293 cells
  • Amino Acid Sequence
    • AccessionP22692
    • SpeciesHuman
    • SequenceDEAIHCPPCS EEKLARCRPP VGCEELVREP GCGCCATCAL GLGMPCGVYT PRCGSGLRCY PPRGVEKPLH TLMHGQGVCM ELAEIEAIQE SLQPSDKDEG DHPNNSFSPC SAHDRRCLQK HFAKIRDRST SGGKMKVNGA PREDARPVPQ GSCQSELHRA LERLAASQSR THEDLYIIPI PNCDRNGNFH PKQCHPALDG QRGKCWCVDR KTGVKLPGGL EPKGELDCHQ LADSFRE
    • Molecular weight26 kDa
    • Amino acids22 to 258
    • TagsHis tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab125753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe ED50 range is 0.01-0.09µg/ml as measured by its ability to inhibit the biological activity of IGFII (20ng/ml) on MCF7 human breast adenocarcinoma cells.
  • Applications

    Functional Studies

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    ab125753 is purified by proprietary chromatographic techniques.
  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C. Store under Desiccating conditions.

    Constituent: 99% PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionIt is recommended to reconstitute ab125753 in sterile ultra pure water not less than 100µg/ml, which can then be further diluted to other aqueous solutions. Upon reconstitution ab125753 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

General Info

  • Alternative names
    • AI875747
    • BP 4
    • BP4
    • Deb2
    • HT29 IGFBP
    • IBP 4
    • IBP-4
    • IBP4
    • IBP4_HUMAN
    • IGF binding protein 4
    • IGF-binding protein 4
    • IGF-BP4
    • IGFBP 4
    • IGFBP-4
    • IGFBP4
    • Insulin Like Growth Factor Binding Protein 4
    • Insulin-like growth factor-binding protein 4
    see all
  • FunctionIGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
  • Sequence similaritiesContains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • Cellular localizationSecreted.
  • Information by UniProt

References for Recombinant human IGFBP4 protein (ab125753)

ab125753 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"