Recombinant Human IKB beta protein (ab85246)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: SDS-PAGE, WB
Description
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Product name
Recombinant Human IKB beta protein
See all IKB beta proteins and peptides -
Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
Purity was determined to be>90% by densitometry.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab85246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Western blot
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
General Info
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Alternative names
- I kappa B beta
- I-kappa-B-beta
- IkappaBbeta
see all -
Function
Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation. -
Tissue specificity
Expressed in all tissues examined. -
Sequence similarities
Belongs to the NF-kappa-B inhibitor family.
Contains 6 ANK repeats. -
Post-translational
modificationsPhosphorylated by RPS6KA1; followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab85246 has not yet been referenced specifically in any publications.