Recombinant Human IKB beta protein (ab85246)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      Purity was determined to be>90% by densitometry.

Specifications

Our Abpromise guarantee covers the use of ab85246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

  • Purity
    > 90 % SDS-PAGE.

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM PMSF, pH 7.5

General Info

  • Alternative names
    • I kappa B beta
    • I-kappa-B-beta
    • IkappaBbeta
    • IKB beta
    • IkB-B
    • IkB-beta
    • IKBB
    • IKBB_HUMAN
    • IkBbeta
    • NF kappa BIB
    • NF-kappa-B inhibitor beta
    • NF-kappa-BIB
    • Nfkbib
    • Thyroid receptor interacting protein 9
    • Thyroid receptor-interacting protein 9
    • TR interacting protein 9
    • TR-interacting protein 9
    • TRIP-9
    • TRIP9
    see all
  • Function
    Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.
  • Tissue specificity
    Expressed in all tissues examined.
  • Sequence similarities
    Belongs to the NF-kappa-B inhibitor family.
    Contains 6 ANK repeats.
  • Post-translational
    modifications
    Phosphorylated by RPS6KA1; followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt

Images

  • SDS-PAGE showing ab85246 at approximately 56kDa.

References

ab85246 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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