Recombinant human IKK alpha protein (ab102103)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • AccessionO15111
    • SpeciesHuman
    • Molecular weight114 kDa including tags
    • Amino acids1 to 745

Associated products

Specifications

Our Abpromise guarantee covers the use of ab102103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySpecific activity is 2.5nm/min/mg
  • Applications

    SDS-PAGE

    Western blot

    Functional Studies

  • Purity> 75 % Densitometry.
    ab102103 was determined to be >75% pure by densitometry.
  • FormLiquid
  • Additional notes

    ab204863 (IKB alpha peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • chuk
    • CHUK1
    • Conserved Helix Loop Helix Ubiquitous Kinase
    • Conserved helix loop ubiquitous kinase
    • Conserved helix-loop-helix ubiquitous kinase
    • I Kappa B Kinase 1
    • I Kappa B Kinase Alpha
    • I-kappa-B kinase 1
    • I-kappa-B kinase alpha
    • IkappaB kinase
    • IkB kinase alpha subunit
    • IkBKA
    • IKK 1
    • IKK A
    • IKK a kinase
    • IKK-A
    • IKK-alpha
    • IKK1
    • IKKA
    • IKKA_HUMAN
    • Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells
    • Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit
    • Inhibitor of nuclear factor kappa-B kinase subunit alpha
    • NFKBIKA
    • Nuclear Factor Kappa B Inhibitor Kinase Alpha
    • Nuclear factor NF kappa B inhibitor kinase alpha
    • Nuclear factor NF-kappa-B inhibitor kinase alpha
    • Nuclear factor NFkappaB inhibitor kinase alpha
    • Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor
    • TCF-16
    • TCF16
    • Transcription factor 16
    see all
  • FunctionActs as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.
  • Tissue specificityWidely expressed.
  • Involvement in diseaseDefects in CHUK are the cause of cocoon syndrome (COCOS) [MIM:613630]; also known as fetal encasement syndrome. COCOS is a lethal syndrome characterized by multiple fetal malformations including defective face and seemingly absent limbs, which are bound to the trunk and encased under the skin.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated.
    Acetylation of Thr-179 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B signaling pathway.
  • Cellular localizationCytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus.
  • Information by UniProt

Recombinant human IKK alpha protein images

  • ab102103 was determined to be >75% pure by densitometry. MWt ~114 kDa.
  • The specific activity of ab102103 was determined to be 2.5 nmol/mg/min.

References for Recombinant human IKK alpha protein (ab102103)

ab102103 has not yet been referenced specifically in any publications.

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