The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 10% Trehalose, 1% Human serum albumin
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
CD121 antigen-like family member A
IL 1R 1
IL 1R alpha
Interleukin 1 receptor alpha type 1
Interleukin 1 receptor antagonist protein (IRAP)
Interleukin 1 receptor type I
Interleukin receptor 1
Interleukin-1 receptor alpha
Interleukin-1 receptor type 1
Interleukin-1 receptor type I
Receptor for interleukin-1 alpha (IL-1A), beta (IL-1B), and interleukin-1 receptor antagonist protein (IL-1RA). Binding to the agonist leads to the activation of NF-kappa-B. Signaling involves formation of a ternary complex containing IL1RAP, TOLLIP, MYD88, and IRAK1 or IRAK2.
Belongs to the interleukin-1 receptor family. Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 TIR domain.
Recombinant Human IL1 Receptor I protein (Fc Chimera) images
Functional Studies - IL1 Receptor I protein (Fc Chimera) (ab83575)
Densitometry of protein isoforms visualised by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.
SDS-PAGE - IL1 Receptor I protein (Fc Chimera) (ab83575)
1D SDS-PAGE of ab83575 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1 – MW markers; Lane 2 – ab83575; Lane 3 – ab83575 treated with PNGase F to remove potential N-linked glycans; Lane 4 – ab83575 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. 10
µg protein loaded per lane. Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes.
References for Recombinant Human IL1 Receptor I protein (Fc Chimera) (ab83575)
has not yet been referenced specifically in any publications.
Publishing research using ab83575? Please let us know so that we can cite the reference in this datasheet.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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