Additional sequence informationA DNA sequence encoding the signal peptide and extracellular domain of human IL-1RI (aa 1-333) was fused to the Fc region of human IgG1 (aa 93-330). Protein expressed in modified human 293 cells.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 10% Trehalose, 1% Human serum albumin
ReconstitutionIt is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
CD121 antigen-like family member A
IL 1R 1
IL 1R alpha
Interleukin 1 receptor alpha type 1
Interleukin 1 receptor antagonist protein (IRAP)
Interleukin 1 receptor type I
Interleukin receptor 1
Interleukin-1 receptor alpha
Interleukin-1 receptor type 1
Interleukin-1 receptor type I
FunctionReceptor for interleukin-1 alpha (IL-1A), beta (IL-1B), and interleukin-1 receptor antagonist protein (IL-1RA). Binding to the agonist leads to the activation of NF-kappa-B. Signaling involves formation of a ternary complex containing IL1RAP, TOLLIP, MYD88, and IRAK1 or IRAK2.
Sequence similaritiesBelongs to the interleukin-1 receptor family. Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 TIR domain.
Recombinant Human IL1 Receptor I protein (Fc Chimera) images
Functional Studies - IL1 Receptor I protein (Fc Chimera) (ab83575)
Densitometry of protein isoforms visualised by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.
SDS-PAGE - IL1 Receptor I protein (Fc Chimera) (ab83575)
1D SDS-PAGE of ab83575 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1 – MW markers; Lane 2 – ab83575; Lane 3 – ab83575 treated with PNGase F to remove potential N-linked glycans; Lane 4 – ab83575 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. 10
µg protein loaded per lane. Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes.
References for Recombinant Human IL1 Receptor I protein (Fc Chimera) (ab83575)
has not yet been referenced specifically in any publications.
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