Recombinant human IL12 p40 protein (ab9993)

Overview

Description

  • NatureRecombinant
  • SourceCHO cells
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceIWELKK DVYVVELDWY PDAPGEMVVL TCDTPEEDGI TWTLDQSSEV LGSGKTLTIQ VKEFGDAGQY TCHKGGEVLS HSLLLLHKKE DGIWSTDILK DQKEPKNKTF LRCEAKNYSG RFTCWWLTTI STDLTFSVKS SRGSSDPQGV TCGAATLSAE RVRGDNKEYE YSVECQEDSA CPAAEESLPI EVMVDAVHKL KYENYTSSFF IRDIIKPDPP KNLQLKPLKN SRQVEVSWEY PDTWSTPHSY FSLTFCVQVQ GKSKREKKDR VFTDKTSATV ICRKNASISV RAQDRYYSSS WSEWASVPCS
    • Molecular weight37 kDa

Associated products

Specifications

Our Abpromise guarantee covers the use of ab9993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Endotoxin level< 0.100 Eu/µg
  • FormLyophilised
  • Additional notesThe ED50, determined by the stimulation of IFN-gamma production by murine splenocytes co-stimulated with IL-12, is < 0.1 ng/ml, corresponding to a specific activity of > 1 x 107 units/mg.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    n/a

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionReconstitute in 1X PBS pH 7.2-7.4 to a concentration of 0.2 mg/ml. This solution can be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for future use.

General Info

  • Alternative names
    • CLMF
    • CLMF p40
    • CLMF2
    • Cytotoxic lymphocyte maturation factor 40 kDa subunit
    • IL 12 subunit p40
    • IL 12B
    • IL-12 subunit p40
    • IL-12B
    • IL12 subunit p40
    • IL12B
    • IL12B_HUMAN
    • interleukin 12 beta chain
    • Interleukin 12 p40
    • Interleukin 12 subunit beta
    • Interleukin 12B
    • Interleukin-12 subunit beta
    • natural killer cell stimulatory factor 40 kD subunit
    • NK cell stimulatory factor chain 2
    • NKSF
    • NKSF2
    • p40
    see all
  • FunctionCytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
    Associates with IL23A to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
  • Involvement in diseaseDefects in IL12B are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity, whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
    Genetic variations in IL12B are a cause of susceptibility to psoriasis type 11 (PSORS11) [MIM:612599]. Psoriasis is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis.
  • Sequence similaritiesBelongs to the type I cytokine receptor family. Type 3 subfamily.
    Contains 1 fibronectin type-III domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Known to be C-mannosylated in the recombinant protein; it is not yet known for sure if the wild-type protein is also modified.
  • Cellular localizationSecreted.
  • Information by UniProt

References for Recombinant human IL12 p40 protein (ab9993)

ab9993 has not yet been referenced specifically in any publications.

Product Wall

Both the human (ab9993) and mouse (ab82106) IL12 proteins are carrier free.


The lab let me know that they did not prepare the sample ourselves, rather we used ab9993 (http://www.abcam.com/index.html?datasheet=9993)

We loaded 10 ng/well and used a 1:5,000 primary dilution. We used our standard SDS-PAGE gel for red...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"