The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Activity was measured by its ability to stimulate the proliferation of PHA-activated human T-lymphoblasts, and is typically in the range of 0.05-0.20 ng/ml.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 10% Trehalose, 1% Human serum albumin
This product is an active protein and may elicit a biological response in vivo, handle with caution.
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
1D SDS-PAGE of ab89013 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab89013
Lane 2: ab89013 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab89013 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
Appearance of additional band at lower MWt after treatment with PNGase F indicates the presence of N-linked glycans. The subsequent drop in MWt after treatment with a glycosidase cocktail indicates the presence of O-linked glycans. Additional high MWt bands in lane 3 are glycosidase enzymes.
SDS-PAGE - IL12 protein (Active) (ab89013)
A sample of ab89013 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. 40 µg of protein was loaded. Spot trains indicate presence of multiple isoforms.
Functional Studies - IL12 protein (Active) (ab89013)
Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational
The triangles indicate theoretical pI and MWt of the alpha and beta chains of the protein.
References for Recombinant human IL12 protein (ab89013)
has not yet been referenced specifically in any publications.
Publishing research using ab89013? Please let us know so that we can cite the reference in this datasheet.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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