The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Endotoxin level< 1.000 Eu/µg
% SDS-PAGE. Greater than 98% by SDS-PAGE and HPLC analyses.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
ReconstitutionCentrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use.
FunctionInduces expression of IL-6, IL-8, and GM-CSF from endothelial cells.
Tissue specificityExpressed preferentially in adipose, skeletal muscle and CNS.