The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Endotoxin level< 0.100 Eu/µg
% SDS-PAGE. Greater than 98% by SDS-PAGE and HPLC analyses.
Endotoxin level is less than 0.1 ng per µg (1EU/µg).
Additional notesThe lyophilized protein is stable at room temperature for
up to 1 month. Working aliquots stored with a carrier
protein are stable for at least 3 months at -20C to -80C.
Avoid repeated freeze/thaw cycles.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Please see notes section.
ReconstitutionCentrifuge the vial prior to opening. Reconstitute in water
to a concentration of 0.1-1.0 mg/ml. Do not vortex. This
solution can be stored at 2-8C for up to 1 week. For
extended storage, it is recommended to further dilute in a
buffer containing a carrier protein (example 0.1% BSA)
and store in working aliquots at -20C to -80C.
FunctionInduces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses.
Tissue specificityExpressed at low levels in several tissues, including brain, kidney, lung, prostate, testis, spinal cord, adrenal gland, and trachea.