The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityActivity :
The activity of IL17F is measured by its ability to induce IL6 production in NHDF cells. The ED50 is typically between 0.04 and 0.1 ng/ml.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C.
Constituents: 10% Trehalose, 1% Human serum albumin, PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Following reconstitution short-term storage at 4°C and longer-term storage of aliquots at -18 to -20°C is recommended. Repeated freeze thawing is not recommended.
Cytokine ML 1
Mutant IL 17F
FunctionLigand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover (PubMed:11591732, PubMed:11591768, PubMed:11574464). Also involved in stimulating the proliferation of peripheral blood mononuclear cells and T-cells and in inhibition of angiogenesis (PubMed:11591732). Plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation.
Tissue specificityExpressed in activated, but not resting, CD4+ T-cells and activated monocytes.
1D SDS-PAGE of ab85600 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab85600
Lane 2: ab85600 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab85600 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
Approximately 5 µg of protein was loaded per lane; Gel was stained using Coomassie.
Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Additional bands in lane 2 and lane 3 are glycosidase enzymes.
References for Recombinant human IL17F protein (ab85600)
has not yet been referenced specifically in any publications.
Publishing research using ab85600? Please let us know so that we can cite the reference in this datasheet.
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