Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceMRPSGRKSSK MQAFRIWDVN QKTFYLRNNQ LVAGYLQGPN VNLEEKIDVV PIEPHALFLG IHGGKMCLSC VKSGDETRLQ LEAVNITDLS ENRKQDKRFA FIRSDSGPTT SFESAACPGW FLCTAMEADQ PVSLTNMPDE GVMVTKFYFQ EDE
    • Amino acids24 to 177

Associated products

Specifications

Our Abpromise guarantee covers the use of ab50081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

    Western blot

  • Endotoxin level< 0.100 Eu/µg
  • Purity> 95 % SDS-PAGE.
    ab50081 purity was assessed also by HPLC. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • FormLyophilised
  • Additional notes


    This protein is the 17.2 kDa secreted isoform of IL1RA.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.

    Preservative: None
    Constituents: 5mM Tris, 25mM Sodium chloride, pH 8

  • ReconstitutionReconstitute to 1mg/ml with distilled water.

General Info

  • Alternative names
    • DIRA
    • ICIL 1RA
    • ICIL-1RA
    • ICIL1RA
    • IL-1ra
    • IL-1ra3
    • IL-1RN
    • IL1 inhibitor
    • IL1F3
    • IL1RA
    • IL1RA_HUMAN
    • IL1RN
    • IL1RN (IL1F3)
    • Interleukin 1 receptor antagonist
    • Interleukin-1 receptor antagonist protein
    • Intracellular IL 1 receptor antagonist type II
    • Intracellular interleukin 1 receptor antagonist (icIL 1ra)
    • IRAP
    • MGC10430
    • MVCD4
    • Type II interleukin 1 receptor antagonist
    see all
  • FunctionInhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
  • Tissue specificityThe intracellular form of IL1RN is predominantly expressed in epithelial cells.
  • Involvement in diseaseMicrovascular complications of diabetes 4
    Interleukin 1 receptor antagonist deficiency
  • Cellular localizationCytoplasm and Secreted.
  • Information by UniProt

Recombinant Human IL1RA protein images

  • Anti-IL1RA antibody (ab2573) at 1 µg/ml + Recombinant Human IL1RA protein (ab50081) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 20 seconds

References for Recombinant Human IL1RA protein (ab50081)

ab50081 has not yet been referenced specifically in any publications.

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