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Source Escherichia coli
Amino Acid Sequence
Sequence MRPSGRKSSK MQAFRIWDVN QKTFYLRNNQ LVAGYLQGPN VNLEEKIDVV PIEPHALFLG IHGGKMCLSC VKSGDETRLQ LEAVNITDLS ENRKQDKRFA FIRSDSGPTT SFESAACPGW FLCTAMEADQ PVSLTNMPDE GVMVTKFYFQ EDE
Amino acids 24 to 177 Specifications
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Endotoxin level < 0.100 Eu/µg
Purity > 95
% SDS-PAGE. ab50081 purity was assessed also by HPLC.
Endotoxin level is less than 0.1 ng per µg (1EU/µg).
This protein is the 17.2 kDa secreted isoform of IL1RA.
Concentration information loading... Preparation and Storage
Stability and Storage
Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.
Constituents: 5mM Tris, 25mM Sodium chloride, pH 8
Reconstitution Reconstitute to 1mg/ml with distilled water.
Function Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
Tissue specificity The intracellular form of IL1RN is predominantly expressed in epithelial cells.
Involvement in disease Microvascular complications of diabetes 4 Interleukin 1 receptor antagonist deficiency
Cellular localization Cytoplasm and Secreted.
Information by UniProt
Recombinant Human IL1RA protein images
Anti-IL1RA antibody ( ab2573) at 1 µg/ml + Recombinant Human IL1RA protein (ab50081) at 0.01 µg Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080) at 1/5000 dilution developed using the ECL technique Performed under reducing conditions. Exposure time : 20 seconds
References for Recombinant Human IL1RA protein (ab50081)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"