The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The ED50 of IL1RA is typically 30-100 ng/ml as measured by its ability to inhibit IL1 mediated proliferation using the murine D10S cell line.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 10% Trehalose, 1% Human serum albumin
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionIt is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
FunctionInhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
Tissue specificityThe intracellular form of IL1RN is predominantly expressed in epithelial cells.
Involvement in diseaseMicrovascular complications of diabetes 4 Interleukin 1 receptor antagonist deficiency
Functional Studies - IL1RA antagonist protein (Active) (ab83761)
Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational
The triangle indicates the theoretical MW and pI of the protein.
SDS-PAGE - IL1RA antagonist protein (Active) (ab83761)
1D SDS-PAGE of ab83761 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab83761
Lane 2: ab83761 treated with PNGase F to remove potential N-linked glycans.
Lane 3: ab83761 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
10 µg protein loaded per lane.
Drop in MW after treatment with PNGase F indicates the presence of N-linked glycans.
Faint bands in lane 3 and lane 4 are glycosidase enzymes.
References for Recombinant human IL1RA protein (ab83761)
has not yet been referenced specifically in any publications.
Publishing research using ab83761? Please let us know so that we can cite the reference in this datasheet.
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