Recombinant human IL2 protein (ab155738)

Overview

Description

  • Nature
    Recombinant
  • Source
    HEK 293 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      APTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFCQSIIS TLT
    • Molecular weight
      15 kDa
    • Amino acids
      21 to 153

Specifications

Our Abpromise guarantee covers the use of ab155738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The bio-activity was determined by dose-dependent stimulation of the proliferation of Mouse CTLL-2 cells.

    The ED50 <0.1ng/ml, corresponding to a specific activity of >1x107 Unit/mg.

  • Applications

    Functional Studies

    SDS-PAGE

  • Endotoxin level
    < 1.000 Eu/µg
  • Purity
    >95% by SDS-PAGE .
    ab155738 was lyophilised from 0.22 µm filtered solution.
  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituents: 95% PBS, 5% Trehalose

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Aldesleukin
    • IL 2
    • IL-2
    • IL2
    • IL2_HUMAN
    • Interleukin 2
    • Interleukin-2
    • Involved in regulation of T cell clonal expansion
    • Lymphokine
    • OTTHUMP00000164090
    • POIL2
    • T Cell Growth Factor
    • T-cell growth factor
    • TCGF
    see all
  • Function
    Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
  • Involvement in disease
    Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.
  • Sequence similarities
    Belongs to the IL-2 family.
  • Cellular localization
    Secreted.
  • Information by UniProt

Images

  • SDS-PAGE analysis of reduced ab155738 stained overnight with Coomassie Blue.

References

ab155738 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
ELISA
IL2 protein (abcam:ab155738) can be used in ELISA.
Coating: IL2 (abcam:ab155738) at 1µg/ml, 0.5µg/ml, 0.25µg/ml
Blocking: 2% Marvel
Primary Ab (starting with 1/100): Anti-IL2 antibody (abcam:ab38151), mouse IgG2a at 2mg/ml
Secondary Ab (1/1000): Goat anti-mouse (Fc specific)-HRP (Sigma A2554).
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Submitted Feb 22 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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