The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The ED50 of ab83684 is typically 0.15- 0.25 ng/ml as measured in a cell proliferation assay using the IL2 dependent murine CTLL2 T cell line.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 10% Trehalose, 1% Human serum albumin, PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Involved in regulation of T cell clonal expansion
T Cell Growth Factor
T-cell growth factor
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
Involvement in disease
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.
Functional Studies - IL2 protein (Active) (ab83684)
Densitometry of protein isoforms visualised by 2-DE. The triangle indicates the theoretical MW and pI of the protein.
SDS-PAGE - IL2 protein (Active) (ab83684)
1D SDS-PAGE of ab83684 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1 ab83684
Lane 2 ab83684 treated with PNGase F to remove potential N-linked glycans
Lane 3 ab83684 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. 10 µg protein
loaded per lane; Deep Purple™ stained.
Drop in MW after treatment with glycosidase cocktail indicates presence of O-linked glycans. Faint bands in lane 3 and lane 4 are glycosidase enzymes.
has not yet been referenced specifically in any publications.
Publishing research using ab83684? Please let us know so that we can cite the reference in this datasheet.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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