SequenceRecombinant Human IL-2 is a 15.5 kDa protein containing 134 amino acid residues including one intrachain disulfide bond:
MAPTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRML TFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNI NVIVLELKGSETTFMCEYAD ETATIVEFLNRWITFAQSIISTLT.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The ED50 as determined by the dose-dependent stimulation of murine CTLL-2 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
Endotoxin level< 0.100 Eu/µg
Additional notesThe ED50, determined by the dose-dependent stimulation of murine CTLL-2 cells, is < 0.1 ng/ml, corresponding to a specific activity of > 1 x 107 units/mg.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Information available upon request.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionCentrifuge the vial prior to opening. Reconstitute in 100 mM Acetic Acid to a concentration of 0.1-1.0 mg/ml. This solution can then be
diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use.
Involved in regulation of T cell clonal expansion
T Cell Growth Factor
T-cell growth factor
FunctionProduced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
Involvement in diseaseNote=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.