The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
< 1.000 Eu/µg
>95% by SDS-PAGE . Determined by SEC-HPLC and reducing SDS-PAGE.
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Selectively expressed in lymphoid tissues. Most highly expressed in thymus and spleen.
Belongs to the type I cytokine receptor family. Type 4 subfamily. Contains 2 fibronectin type-III domains.
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box 1 motif is required for JAK interaction and/or activation.
C-mannosylated at Trp-214 in the WSXWS motif, the sugar chain makes extensive hydrogen bonds with Asn-73 sugar, and bridges the two fibronectin domains transforming the V-shaped receptor into an A-frame.