Overview

Description

  • Nature
    Recombinant
  • Source
    HEK 293 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      75 kDa including tags
    • Amino acids
      20 to 189
    • Tags
      DDDDK tag N-Terminus
    • Additional sequence information
      Human IL-12 p40 peptide (aa 23-328) is fused at the C-terminus to a 18 aa linker peptide (GSTSGSGKPGSGEGSTKG) and to the IL23 p19 peptide (aa 20-189) and tagged at the N-terminus to a DDDDK-tag.

Specifications

Our Abpromise guarantee covers the use of ab109153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Induction of IL-17 secretion by mouse splenocytes.

  • Applications

    Functional Studies

    SDS-PAGE

  • Endotoxin level
    < 0.100 Eu/µg
  • Purity
    > 90 % SDS-PAGE.
    ab109153 is 0.2 µm filtered.
  • Form
    Liquid
  • Additional notes
    Induction of IL17 secretion by Mouse splenocytes.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Constituent: 99% PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • IL 23
    • IL 23 A
    • IL 23 subunit alpha
    • IL 23A
    • IL 23p19
    • IL-23 subunit alpha
    • IL-23-A
    • IL-23p19
    • IL12B
    • IL23
    • Il23a
    • IL23A_HUMAN
    • IL23P19
    • interleukin 12B
    • Interleukin 23 alpha subunit p19
    • Interleukin 23 p19 subunit
    • interleukin 23 subunit alpha
    • interleukin 23 subunit p19
    • interleukin six, G CSF related factor
    • Interleukin-23 subunit alpha
    • Interleukin-23 subunit p19
    • JKA3 induced upon T cell activation
    • MGC79388
    • P19
    • SGRF
    see all
  • Function
    Associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
  • Tissue specificity
    Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level).
  • Sequence similarities
    Belongs to the IL-6 superfamily.
  • Developmental stage
    Expressed by newborns dendritic cells.
  • Cellular localization
    Secreted. Secreted upon association with IL12B.
  • Information by UniProt

Images

  • IL17 secretion by Mouse splenocytes
    Mononuclear cells isolated from Mouse spleen were cultured with Mouse IL23 in presence or absence of various concentrations of ab109153 for 3 days. Levels of IL17 accumulated in culture supernatants were measured using ELISA.

References

ab109153 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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