Recombinant human IL31 protein (ab106619)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ6EBC2
    • SpeciesHuman
    • SequenceSHTLPVRLLR PSDDVQKIVE ELQSLSKMLL KDVEEEKGVL VSQNYTLPCL SPDAQPPNNI HSPAIRAYLK TIRQLDNKSV IDEIIEHLDK LIFQDAPETN ISVPTDTHEC KRFILTISQQ FSECMDLALK SLTSGAQQAT T
    • Molecular weight16 kDa
    • Amino acids24 to 164

Specifications

Our Abpromise guarantee covers the use of ab106619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityMeasured by its ability to induce STAT3 activation in Human U87 cells.
  • Applications

    SDS-PAGE

    HPLC

    ELISA

    Western blot

  • Endotoxin level< 0.100 Eu/µg
  • Purity> 98 % SDS-PAGE.
    Greater than 98% by SDS-PAGE gel and HPLC analyses
  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionThe lyophilised product should be stored at -20oC till use. Once the product has been reconstituted according to the CoA, this can be diluted further in a chosen aqueous buffer containing a carrier protein, such as 0.1% BSA and stored in working aliquots at -20oC for at least 3 months

General Info

  • Alternative names
    • IL 31
    • IL-31
    • IL31
    • IL31_HUMAN
    • Interleukin 31
    • Interleukin-31
    see all
  • FunctionActivates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity.
  • Tissue specificityDetected at low levels in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea.
  • Cellular localizationSecreted.
  • Information by UniProt

Recombinant human IL31 protein images

References for Recombinant human IL31 protein (ab106619)

ab106619 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application ELISA

Sample: Human Serum (human serum samples)
Specification: human serum samples
Blocking step: BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Type: Direct

Other product details
Dilution: 1/30000
Incubation time: 2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS with 0,1%BSA and 0,05%Tween20

Primary antibody (in addition to 'IL31 protein')
Name: Non-Abcam antibody was used: monoclonal anti IL-31
Host species: Mouse
Clonality: Monoclonal
Dilution: 1/250

Secondary antibody
Name: Non-Abcam antibody was used: monoclonal anti IL31
Host species: Mouse
Clonality: Monoclonal
Conjugation: Biotin
Dilution: 1/2000

Additional data
Additional Notes: IL-31 used as standard
range: 3-300pg/ml
Username

Abcam user community

Verified customer

Submitted Jan 21 2013

Application Western blot

Sample: Human Cell lysate - whole cell (eosinophil granulocyte)
Loading amount: 500000 cells
Specification: eosinophil granulocyte
Gel Running Conditions: Reduced Denaturing (5-16%)
Blocking step: BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details
Dilution: 1/200
Incubation time: 14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA / TBS

Primary antibody (in addition to 'IL31 protein')
Dilution: 1/100

Secondary antibody
Name: Non-Abcam antibody was used: anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution: 1/2000

Detection
Detection method: Chemiluminescence
Exposure: 45 minute(s) and 0 second(s)
Bands: Specific: 18 kDa Non-specific: - kDa
Positive control: stimulation with IL-3 (10ng/ml) + IL-5 (10ng/ml) + GM-CSF (10ng/ml) for 30 minutes
Negative control: non stimulated cells
Username

Abcam user community

Verified customer

Submitted Dec 07 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"