The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Measured by its ability to induce STAT3 activation in Human U87 cells.
< 0.100 Eu/µg
% SDS-PAGE. Greater than 98% by SDS-PAGE gel and HPLC analyses
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
This product is an active protein and may elicit a biological response in vivo, handle with caution.
The lyophilised product should be stored at -20oC till use. Once the product has been reconstituted according to the CoA, this can be diluted further in a chosen aqueous buffer containing a carrier protein, such as 0.1% BSA and stored in working aliquots at -20oC for at least 3 months
Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity.
Detected at low levels in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea.
Western blot - IL31 protein (ab106619)Image courtesy of an anonymous Abreview.
Whole cell lysate prepared from human eosinophil granulocytes was loaded at 500000 cells. 5-16% gel run under reducing, denaturing conditions.Blocking step performed using 5% BSA for 2 hours at 25°C. ab106619 used at a 1/200 dilution.Primary antibody used at a 1/100 dilution. Secondary antibody was an HRP conjugated goat anti-rabbit IgG, used at a 1/200 dilution.The positive control was stimulated with IL-3 (10ng/ml) + IL-5 (10ng/ml) + GM-CSF (10ng/ml) for 30 minutes.