The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 10% Trehalose, 1% Human serum albumin
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
IL 3 receptor alpha SP2 isoform
IL 3R alpha
IL-3 receptor subunit alpha
IL-3R subunit alpha
IL3 Receptor alpha
Interleukin 3 receptor alpha chain
Interleukin 3 receptor, alpha
Interleukin 3 receptor, alpha (low affinity)
Interleukin-3 Receptor alpha
Interleukin-3 receptor subunit alpha
Interleukin3 receptor, Y-chromosomal
This is a receptor for interleukin-3.
Belongs to the type I cytokine receptor family. Type 5 subfamily.
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box 1 motif is required for JAK interaction and/or activation.
1D SDS-PAGE of ab88358 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab88358
Lane 2: ab88358 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab88358 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
Drop in MWt after treatment with PNGase F indicates presence of N-linked glycans. Subsequent drop in MWt after treatment with glycosidase cocktail indicates presence of O-linked glycans. Faint bands in lane 2 and lane 3 are glycosidase enzymes.
SDS-PAGE - IL3RA protein (Fc Chimera) (ab88358)
A sample of ab88358 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. Spot train indicates presence of multiple isoforms of ab88358.
Functional Studies - IL3RA protein (Fc Chimera) (ab88358)
Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational
The triangle indicates the theoretical MWt and pI of the protein.
has not yet been referenced specifically in any publications.
Publishing research using ab88358? Please let us know so that we can cite the reference in this datasheet.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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