The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 10% Trehalose, 1% Human serum albumin, PBS
ReconstitutionIt is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
FunctionHigh affinity receptor for interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor.
Sequence similaritiesBelongs to the type I cytokine receptor family. Type 4 subfamily. Contains 2 fibronectin type-III domains.
DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box 1 motif is required for JAK interaction and/or activation.
Recombinant Human IL3RB protein (Fc Chimera) images
Functional Studies - IL3RB– Fc Chimera (ab83919)
Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified Human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational
The triangle indicates the theoretical MW and pI of the protein.
SDS-PAGE - IL3RB– Fc Chimera (ab83919)
1D SDS-PAGE of ab83919 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab83919
Lane 2: ab83919 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab83919 treated with a glycosidase cocktail to
remove potential N- and O-linked glycans.
Approximately 5 μg of protein was loaded per lane; Gel was stained using Deep Purple™.
References for Recombinant Human IL3RB protein (Fc Chimera) (ab83919)
has not yet been referenced specifically in any publications.
Publishing research using ab83919? Please let us know so that we can cite the reference in this datasheet.
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