The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C.
Constituents: 10% Trehalose, 1% Human serum albumin
ReconstitutionIt is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Lane 1 – MW markers; Lane 2 – ab83941; Lane 3 – ab83941 treated with PNGase F to remove potential N-linked glycans; Lane 4 – ab83941 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. 10 ug protein loaded per lane. Deep Purple stained. Appearance of two bands after treatment with PNGase F indicates presence of N-linked glycans. Subsequent drop in MW of top band after treatment with glycosidase cocktail indicates presence of O-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes.
Functional Studies - IL5 protein (ab83941)
Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification. The triangle indicates theoretical pI and MW of theprotein.
References for Recombinant Human IL5 protein (ab83941)
has not yet been referenced specifically in any publications.
Publishing research using ab83941? Please let us know so that we can cite the reference in this datasheet.
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