Recombinant human Insulin protein (ab123768)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP01308
    • SpeciesHuman
    • SequenceGIVEQCCTSIC SLYQLENYCN FVNQHL CGSHLVEALY LVCGERGFFY TPKT
    • Molecular weight6 kDa
    • Additional sequence informationRecombinant Human Insulin produced in E. coli is a two chain, non-glycosylated polypeptide chain containing 51 amino acids and having a molecular mass of 5.81 kDa.

Specifications

Our Abpromise guarantee covers the use of ab123768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityab123768 is fully biologically active when compared to World Health Organization (WHO) reference standard which is 28 units/mg.
  • Applications

    Functional Studies

    SDS-PAGE

    HPLC

  • Purity> 98 % SDS-PAGE.
    Purity is greater than 98% as determined by HPLC and SDS-PAGE. ab123768 is purified by proprietary chromatographic techniques. Endotoxin Level: <0.1 ng/µg of Insulin.
  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C. Store under Desiccating conditions.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionReconstitute in 5-10 mM HCl to a concentration of 1.0 mg/ml. The solution can then be diluted to other aqueous buffers. Upon reconstitution ab123768 should be stored at 4°C for 2-7 days. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA) and store aliquots at –20°C or –70°C. Avoid freeze-thaw cycles.

General Info

  • Alternative names
    • IDDM
    • IDDM1
    • IDDM2
    • ILPR
    • ins
    • INS_HUMAN
    • Insulin A chain
    • Insulin B chain
    • IRDN
    • MODY10
    • Preproinsulin
    • Proinsulin
    • Proinsulin precursor
    see all
  • FunctionInsulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • Involvement in diseaseDefects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
    Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
    Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similaritiesBelongs to the insulin family.
  • Cellular localizationSecreted.
  • Information by UniProt

References for Recombinant human Insulin protein (ab123768)

ab123768 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application ELISA
Sandwich ELISA was set up using human purified insulin for standard curve, set up from 1.5μg/mL protein in a 1:2 serial dilution. Ab14042 used at 2μg/mL as capture antibody and Ab6995 used at 1:50 in a 1:4 serial dilution used for the detection antibody. Anti-mouse HRP conjugated secondary antibody used at 1:20,000.
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Ms. Vruti Patel

Verified customer

Submitted Dec 17 2014

We do offer a number of active insulin proteins for use as standard including http://www.abcam.com/insulin-protein-active-ab73797.html (25mg size) or http://www.abcam.com/insulin-protein-active-ab123768.html(5mg size). Abcam also carries a number of...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"