The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Additional notesProtein concentration is above or equal to 0.05 mg/ml. Best use within three months from the date of receipt of this protein.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00 Constituents: 0.3% Glutathione, 0.79% Tris HCl
B4 integrin interactor
Binding protein of beta-4 integrin
Eukaryotic translation initiation factor 3A
Eukaryotic translation initiation factor 6
p27 beta 4 integrin binding protein
FunctionBinds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May behave as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (GNB2L1)-dependent protein kinase C activity.
Tissue specificityExpressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level).
Sequence similaritiesBelongs to the eIF-6 family.
Post-translational modificationsPhosphorylation at Ser-174 and Ser-175 promotes nuclear export.
Cellular localizationCytoplasm. Nucleus > nucleolus. Shuttles between cytoplasm and nucleus/nucleolus.