Recombinant human Interferon alpha 2 protein (ab187225)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 98% SDS-PAGE
- Endotoxin level: <= 1.000 Eu/µg
- Active: Yes
- Suitable for: HPLC, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human Interferon alpha 2 protein
See all Interferon alpha 2 proteins and peptides -
Biological activity
Activity is determined by the ability induce antiviral activity. This is determined to be ≥ 2.0 x 10^8 U/mg determined by Viral CPE assay using EMC virus on A549 cells.
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Purity
>= 98 % SDS-PAGE.
Purity determined by HPLC, Reducing and Non-reducing SDS-PAGE -
Endotoxin level
<=1.000 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MCDLPQTHSL GSRRTLMLLA QMRKISLFSC LKDRHDFGFP QEEFGNQFQK AETIPVLHEM IQQIFNLFST KDSSAAWDET LLDKFYTELY QQLNDLEACV IQGVGVTETP LMKEDSILAV RKYFQRITLY LKEKKYSPCA WEVVRAEIMR SFSLSTNLQE SLRSKE -
Predicted molecular weight
19 kDa -
Amino acids
24 to 188 -
Additional sequence information
Non-glycosylated.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab187225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
HPLC
Functional Studies
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon reconsitution add a carrier protein (0.1% BSA). Store at -20°C long term. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionCentrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilised product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
General Info
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Alternative names
- Alpha 2a interferon
- IFN
- IFN alpha 2
see all -
Function
Produced by macrophages, IFN-alpha have antiviral activities. -
Sequence similarities
Belongs to the alpha/beta interferon family. -
Cellular localization
Secreted. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab187225 has not yet been referenced specifically in any publications.