The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The biological activity of ab168091 was measured in an inhibition assay using the Human K562 cells. The ED50 for this effect is typically 400 - 600 ng/mL.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20ºC.
Constituents: 89% PBS, 10% Trehalose
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Reconstitute with sterile phosphate-buffered saline containing at least 0.1% carrier protein. Store in working aliquots with a carrier protein at -20°C. Avoid repeated freeze/thaw cycles.
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Released primarily from activated T lymphocytes.
Involvement in disease
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
Belongs to the type II (or gamma) interferon family.
Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.