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Our Abpromise guarantee covers the use of ab42621 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Specific Activity : 8 pmol/min/μg. One unit defined as the amount of enzyme that will transfer 1nmol phosphate to Tyr substrate per minute at pH 7.4 and 30°C.
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Lane 1: 1.4μg ab42621
Lane 2: Protien Marker
Observed weight: 98 kDa
Enzyme reaction is conducted in a buffer containing 50 mM HEPES, pH 7.5, 10 mM MgCl2, 1 mM EGTA, 200 μM ATP, 0.01% Brij-35, 2 μM substrate (Tyr Peptide 6, Z-lyte kinase assay kit from Invitrogen), and 0-100 ng enzyme at 37°C for 1 hour.
ab42621 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"