Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Tags
      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab45153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

General Info

  • Alternative names
    • c Jun kinase 3
    • c-Jun N-terminal kinase 3
    • cJun N terminal kinase 3
    • FLJ12099
    • FLJ33785
    • JNK3
    • JNK3 alpha protein kinase
    • JNK3A
    • MAP kinase
    • MAP kinase 10
    • MAP kinase p49 3F12
    • MAPK 10
    • Mapk10
    • MGC50974
    • mitogen activated protein kinase 10
    • Mitogen-activated protein kinase 10
    • MK10_HUMAN
    • p493F12
    • p54bSAPK
    • PRKM10
    • protein kinase mitogen activated 10
    • SAPK1b
    • Stress activated protein kinase 1b
    • stress activated protein kinase beta
    • Stress activated protein kinase JNK3
    • Stress-activated protein kinase JNK3
    see all
  • Function
    Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. Required for stress-induced neuronal apoptosis and the pathogenesis of glutamate excitotoxicity.
  • Tissue specificity
    Specific to a subset of neurons in the nervous system. Present in the hippocampus and areas, cerebellum, striatum, brain stem, and weakly in the spinal cord. Very weak expression in testis and kidney.
  • Involvement in disease
    Defects in MAPK10 are a cause of epileptic encephalopathy Lennox-Gastaut type (EELG) [MIM:606369]. Epileptic encephalopathies of the Lennox-Gastaut group are childhood epileptic disorders characterized by severe psychomotor delay and seizures. Note=A chromosomal aberration involving MAPK10 has been found in a single patient. Translocation t(Y;4)(q11.2;q21) which causes MAPK10 truncation.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-221 and Tyr-223, which activates the enzyme. Weakly autophosphorylated on threonine and tyrosine residues in vitro.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

Images

References

ab45153 has not yet been referenced specifically in any publications.

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