The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityHuman Kallikrein 1 active. Unit Definitin: 1 unit will hydrolize 1 umole of H-D-Val-Leu-Arg-pNA per minute at pH 8.0 at 37°C Human Kallikrein 1 measured biological activity of no less than 5 Units/mg
% SDS-PAGE. ab117200 is purified by proprietary chromatographic techniques.
Additional notesalthough stable at 4°C for 3 weeks, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituent: 100% PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionIt is recommended to reconstitute the lyophilized KLK1 in sterile 18MO-cm H2O not less than 100 µg/ml or more than 10 mg/ml solutions.
Glandular kallikrein 1
Kallikrein serine protease 1
Kidney/pancreas/salivary gland kallikrein
FunctionGlandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
Tissue specificityIsoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84.
Sequence similaritiesBelongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.
Post-translational modificationsThe O-linked polysaccharides on Ser-93, Ser-104 and Ser-167 are probably the mucin type linked to GalNAc. In PubMed:3163150, GalNAc was detected with the corresponding peptides but not located.