Recombinant human Kallikrein 1 protein (ab117200)

Overview

Description

  • Nature
    Recombinant
  • Source
    Yeast
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      28 kDa
    • Amino acids
      25 to 262

Specifications

Our Abpromise guarantee covers the use of ab117200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Human Kallikrein 1 active. Unit Definitin: 1 unit will hydrolize 1 umole of H-D-Val-Leu-Arg-pNA per minute at pH 8.0 at 37°C Human Kallikrein 1 measured biological activity of no less than 5 Units/mg
  • Applications

    HPLC

    SDS-PAGE

  • Purity
    > 90 % SDS-PAGE.
    ab117200 is purified by proprietary chromatographic techniques.
  • Form
    Lyophilised
  • Additional notes
    although stable at 4°C for 3 weeks, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Constituent: 100% PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized KLK1 in sterile 18MO-cm H2O not less than 100 µg/ml or more than 10 mg/ml solutions.

General Info

  • Alternative names
    • Glandular kallikrein 1
    • hK 1
    • hK1
    • Kallikrein serine protease 1
    • Kallikrein-1
    • Kidney/pancreas/salivary gland kallikrein
    • Klk 6
    • KLK1
    • KLK1_HUMAN
    • Klk6
    • KLKR
    • Tissue kallikrein
    see all
  • Function
    Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
  • Tissue specificity
    Isoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84.
  • Sequence similarities
    Belongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications
    The O-linked polysaccharides on Ser-93, Ser-104 and Ser-167 are probably the mucin type linked to GalNAc. In PubMed:3163150, GalNAc was detected with the corresponding peptides but not located.
  • Information by UniProt

References

ab117200 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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