Recombinant Human KAT13A / SRC1 protein (ab82062)

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Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
      Q15788
    • Species
      Human
    • Amino acids
      627 to 786
    • Additional sequence information
      This protein contains the Tobacco Etch virus (TEV) protease site & can be cleaved using acTEV to yield untagged SRC1. This protein will be useful for assays where a native untagged protein is desired.

Specifications

Our Abpromise guarantee covers the use of ab82062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.
    ab82062 is purified by an affinity column.
  • Form
    Liquid
  • Additional notes

    ab82062 contains the Tobacco Etch virus (TEV) protease site and can be cleaved using acTEV to yield untagged KAT13A / SRC1 receptor interacting domain (RID). This protein will be useful for assays where a native untagged protein is desired.

    ab82062 can also be used for protein-protein interaction assays. 100 units are sufficient for a protein-protein interaction assay. 1 unit equals 1 nanogram of purified protein.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 20mM Tris HCl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0

General Info

  • Alternative names
    • bHLHe74
    • Class E basic helix-loop-helix protein 74
    • F SRC 1
    • Hin 2 protein
    • Hin2 protein
    • MGC129719
    • MGC129720
    • mNRC 1
    • NCoA 1
    • NCoA-1
    • Ncoa1
    • NCOA1_HUMAN
    • Nuclear receptor coactivator 1
    • Nuclear receptor coactivator protein 1
    • NY REN 52 antigen
    • Protein Hin 2
    • Protein Hin-2
    • Protein Hin2
    • Renal carcinoma antigen NY REN 52
    • Renal carcinoma antigen NY-REN-52
    • RIP 160
    • RIP160
    • SRC 1
    • SRC-1
    • Steroid receptor coactivator 1
    see all
  • Function
    Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3.
  • Tissue specificity
    Widely expressed.
  • Involvement in disease
    Note=A chromosomal aberration involving NCOA1 is a cause of rhabdomyosarcoma. Translocation t(2;2)(q35;p23) with PAX3 generates the NCOA1-PAX3 oncogene consisting of the N-terminus part of PAX3 and the C-terminus part of NCOA1. The fusion protein acts as a transcriptional activator. Rhabdomyosarcoma is the most common soft tissue carcinoma in childhood, representing 5-8% of all malignancies in children.
  • Sequence similarities
    Belongs to the SRC/p160 nuclear receptor coactivator family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAS (PER-ARNT-SIM) domain.
  • Domain
    The C-terminal (1107-1441) part mediates the histone acetyltransferase (HAT) activity.
    Contains 7 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. LXXLL motifs 3, 4 and 5 are essential for the association with nuclear receptors. LXXLL motif 7, which is not present in isoform 2, increases the affinity for steroid receptors in vitro.
  • Post-translational
    modifications
    Sumoylated; sumoylation increases its interaction with PGR and prolongs its retention in the nucleus. It does not prevent its ubiquitination and does not exert a clear effect on the stability of the protein.
    Ubiquitinated; leading to proteasome-mediated degradation. Ubiquitination and sumoylation take place at different sites.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt

References

ab82062 has not yet been referenced specifically in any publications.

Publishing research using ab82062? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1 Review or Q&A

10
Votes: Highest First

Dear technical team,

Our customer would like to purchase ab82602 and it is His tagged recombinant protein.
She would like to know where His were tagged on, N-terminal or C-terminal.
Please let me know.

Best regards

Thank you for your inquiry.

The product number ab82602 is a antibody.

I suspect a typo. The products numbers ab82062 (KAT13A / SRC1 protein (His tag))and ab82206 (p53 protein (His tag))are both proteins.

I am happy to conf...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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