Recombinant Human KAT2B / PCAF protein (ab56275)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      69 kDa
    • Amino acids
      431 to 832
    • Tags
      GST tag N-Terminus
    • Additional sequence information
      Recombinant fragment, corresponding to amino acids 431-end of Human PCAF.

Specifications

Our Abpromise guarantee covers the use of ab56275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form
    Liquid
  • Additional notes


    PCAF is a binding protein that associates itself with p300/CBP and has no enzymatic activity. However storage at -80C and in glycerol is considered to prevent denaturation and to ensure that its binding conformation is conserved.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM PMSF, pH 7.5

General Info

  • Alternative names
    • CAF
    • CREBBP associated factor
    • GCN5
    • GCN5L
    • GCN5L1
    • Histone acetylase PCAF
    • Histone acetyltransferase KAT2B
    • Histone acetyltransferase PCAF
    • K(lysine) acetyltransferase 2B
    • KAT2B
    • KAT2B_HUMAN
    • Lysine acetyltransferase 2B
    • P
    • P/CAF
    • p300/CBP associated factor
    • P300/CBP-associated factor
    • Pcaf
    see all
  • Function
    Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes.
  • Tissue specificity
    Ubiquitously expressed but most abundant in heart and skeletal muscle.
  • Sequence similarities
    Belongs to the GCN5 family.
    Contains 1 bromo domain.
    Contains 1 N-acetyltransferase domain.
  • Domain
    The bromodomain mediates binding to HIV-1 Tat.
  • Cellular localization
    Nucleus.
  • Information by UniProt

Images

  • ab56275 on SDS-PAGE. The extra band found on the SDS gel may be a truncated form of PCAF, but because we have not yet done a Western Blot on this product, the identity is still unconfirmed.

References

ab56275 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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