The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
PCAF is a binding protein that associates itself with p300/CBP and has no enzymatic activity. However storage at -80C and in glycerol is considered to prevent denaturation and to ensure that its binding conformation is conserved.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
FunctionFunctions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes.
Tissue specificityUbiquitously expressed but most abundant in heart and skeletal muscle.
Sequence similaritiesBelongs to the GCN5 family. Contains 1 bromo domain. Contains 1 N-acetyltransferase domain.
DomainThe bromodomain mediates binding to HIV-1 Tat.