Recombinant Human KDM1 / LSD1 protein (ab125649)



  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • AccessionO60341
    • SpeciesHuman
    • Molecular weight100 kDa including tags
    • Amino acids172 to 852


Our Abpromise guarantee covers the use of ab125649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot


  • Purity> 95 % Densitometry.

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.005% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

General Info

  • Alternative names
    • Amine oxidase (flavin containing) domain 2
    • Amine oxidase, flavin containing, 2
    • AOF2
    • BHC110
    • BRAF35 HDAC complex protein BHC110
    • BRAF35-HDAC complex protein BHC110
    • BRAF35/HDAC complex, 110-kD subunit
    • CPRF
    • EC1
    • FAD binding protein BRAF35 HDAC complex, 110 kDa subunit
    • Flavin-containing amine oxidase domain-containing protein 2
    • KDM 1
    • KDM1
    • Kdm1a
    • KIAA0601
    • LSD 1
    • LSD1
    • Lysine (K) specific demethylase 1
    • Lysine (K) specific demethylase 1A
    • Lysine demethylase 1A
    • Lysine specific histone demethylase 1
    • Lysine specific histone demethylase 1A
    • Lysine-specific demethylase 1
    • Lysine-specific demethylase 1A
    • Lysine-specific histone demethylase 1A
    see all
  • FunctionHistone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the flavin monoamine oxidase family.
    Contains 1 SWIRM domain.
  • DomainThe SWIRM domain may act as an anchor site for a histone tail.
  • Cellular localizationNucleus.
  • Information by UniProt

Recombinant Human KDM1 / LSD1 protein images

  • SDS-PAGE analysis of ab125649.

References for Recombinant Human KDM1 / LSD1 protein (ab125649)

ab125649 has not yet been referenced specifically in any publications.

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