Recombinant Human KDM4C / GASC1 / JMJD2C protein (ab167940)

Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MEVAEVESPL NPSCKIMTFR PSMEEFREFN KYLAYMESKG AHRAGLAKVI PPKEWKPRQC YDDIDNLLIP APIQQMVTGQ SGLFTQYNIQ KKAMTVKEFR QLANSGKYCT PRYLDYEDLE RKYWKNLTFV APIYGADING SIYDEGVDEW NIARLNTVLD VVEEECGISI EGVNTPYLYF GMWKTTFAWH TEDMDLYSIN YLHFGEPKSW YAIPPEHGKR LERLAQGFFP SSSQGCDAFL RHKMTLISPS VLKKYGIPFD KITQEAGEFM ITFPYGYHAG FNHGFNCAES TNFATVRWID YGKVAKLCTC RKDMVKISMD IFVRKFQPDR YQLWKQGKDI YTIDHTKPTP ASTPEVKAWL QRRRKVRKAS RSFQCARSTS KRPKADEEEE VSDEVDGAEV PNPDSVTDDL KVSEKSEAAV KLRNTEASSE EESSASRMQV EQNLSDHIKL SGNSCLSTSV
    • Molecular weight
      82 kDa including tags
    • Amino acids
      1 to 460
    • Tags
      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab167940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity
    > 95 % Densitometry.

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.004% EDTA, 25% Glycerol, 0.29% Sodium chloride

General Info

  • Alternative names
    • bA146B14.1
    • GASC 1 protein
    • GASC-1 protein
    • GASC1
    • Gene amplified in squamous cell carcinoma 1 protein
    • JHDM3C
    • JmjC domain containing histone demethylation protein 3C
    • JmjC domain-containing histone demethylation protein 3C
    • JMJD2C
    • Jumonji domain containing 2C
    • Jumonji domain containing protein 2C
    • Jumonji domain-containing protein 2C
    • Kdm4c
    • KDM4C_HUMAN
    • Lysine (K) specific demethylase 4C
    • Lysine demethylase 4C
    • Lysine-specific demethylase 4C
    • TDRD14C
    • Tudor domain containing 14C
    see all
  • Function
    Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate.
  • Tissue specificity
    Overexpressed in several esophageal squamous cell carcinomas (ESCs).
  • Sequence similarities
    Belongs to the JHDM3 histone demethylase family.
    Contains 1 JmjC domain.
    Contains 1 JmjN domain.
    Contains 2 PHD-type zinc fingers.
    Contains 2 Tudor domains.
  • Domain
    The 2 Tudor domains recognize and bind methylated histones. Double Tudor domain has an interdigitated structure and the unusual fold is required for its ability to bind methylated histone tails.
  • Cellular localization
    Nucleus.
  • Information by UniProt

Images

  • SDS Page analysis of ab167940

References

This product has been referenced in:
  • Jin C  et al. Chem-seq permits identification of genomic targets of drugs against androgen receptor regulation selected by functional phenotypic screens. Proc Natl Acad Sci U S A 111:9235-40 (2014). WB . Read more (PubMed: 24928520) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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