Recombinant Human KLF4 protein (ab91256)

Overview

Description

  • Nature
    Recombinant
  • Source
    HEK 293 cells
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MAVSDALLPS FSTFASGPAG REKTLRQAGA PNNRWREELS HMKRLPPVLP GRPYDLAAAT VATDLESGGA GAACGGSNLA PLPRRETEEF NDLLDLDFIL SNSLTHPPES VAATVSSSAS ASSSSSPSSS GPASAPSTCS FTYPIRAGND PGVAPGGTGG GLLYGRESAP PPTAPFNLAD INDVSPSGGF VAELLRPELD PVYIPPQQPQ PPGGGLMGKF VLKASLSAPG SEYGSPSVIS VSKGSPDGSH PVVVAPYNGG PPRTCPKIKQ EAVSSCTHLG AGPPLSNGHR PAAHDFPLGR QLPSRTTPTL GLEEVLSSRD CHPALPLPPG FHPHPGPNYP SFLPDQMQPQ VPPLHYQELM PPGSCMPEEP KPKRGRRSWP RKRTATHTCD YAGCGKTYTK SSHLKAHLRT HTGEKPYHCD WDGCGWKFAR SDELTRHYRK HTGHRPFQCQ KCDRAFSRSD HLALHMKRHF GGYGRKKRRQ RRR
    • Molecular weight
      52 kDa
    • Amino acids
      10 to 479
    • Additional sequence information
      ab91256 is a 483 amino acid protein, including a 13-residue C- term TAT peptide. ab91256 is a mixture of the expected sequence beginning at Met1 and a truncated isoform beginning at Tyr54.

Specifications

Our Abpromise guarantee covers the use of ab91256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Endotoxin level
    < 0.100 Eu/µg
  • Purity
    > 90 % SDS-PAGE.
    Purity greater than 90% by SDS-PAGE gel and HPLC analyses
  • Form
    Lyophilised
  • Additional notes
    Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C.

  • Reconstitution
    Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C; for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C; to -80°C;

General Info

  • Alternative names
    • Endothelial Kruppel like zinc finger protein
    • Epithelial zinc finger protein EZF
    • EZF
    • GKLF
    • gut Kruppel-like factor
    • Gut-enriched krueppel-like factor
    • KLF
    • KLF4
    • KLF4_HUMAN
    • Krueppel-like factor 4
    • Kruppel like factor 4 (Epithelial zinc finger protein EZF) (Gut enriched Krueppel like factor)
    • Kruppel like factor 4 (gut)
    see all
  • Function
    Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Domain
    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Cellular localization
    Nucleus.
  • Information by UniProt

References for Recombinant Human KLF4 protein (ab91256)

ab91256 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.
The activity of our KLF4 proteins has not been tested, so we do not know whether they are active or inactive. We do have discounts for buying 10 units or more.

I hope this information is helpful to you. Plea...

Read More

Thank you for your inquiry.

I can confirm that ab91256 (KLF4 protein) has a 13- residue C-terminal TAT tag.

Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up