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Our Abpromise guarantee covers the use of ab80289 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Specific Activity: 3.5 pmol/min/μg.
Assay conditions: 50 μl reaction mix (50 mM TRIS pH 8.8, 5mM MgCl2, 4mM DTT, 0.05% Tween-20, 40 μM S-adenosylmethionine, and 10-100 ng SUV39H1) add to the wells coated with the substrate on a GBioscience Neutravidin black plate. Incubate for 1 hr. Add antibody against methylated K9 residue of histone H3, incubate 1 hr. Then, add secondary HRP-labeled antibody and
incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence.
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 30% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Image showing specific activity of ab80289.
10% SDS-PAGE showing ab80289 at approximately 64kDa (3µg). 2μg of protein loaded.
ab80289 has not yet been referenced specifically in any publications.