• NatureRecombinant
  • SourceWheat germ
  • Amino Acid Sequence
    • AccessionO00505
    • SpeciesHuman
    • Molecular weight37 kDa including tags
    • Amino acids422 to 521


Our Abpromise guarantee covers the use of ab114747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications



    Western blot

  • FormLiquid
  • Additional notesProtein concentration is above or equal to 0.05 mg/ml.
    Best used within three months from the date of receipt.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names
    • SRP1 gamma
    • hSRP1
    • IMA4_HUMAN
    • Importin alpha 3
    • Importin alpha 3 subunit
    • Importin alpha 4
    • Importin alpha Q2
    • Importin subunit alpha-4
    • IPOA4
    • Karyopherin alpha 3
    • Karyopherin alpha 3 importin alpha 4
    • Karyopherin alpha 3 subunit
    • Karyopherin subunit alpha 3
    • Karyopherin subunit alpha-3
    • KPNA 3
    • Kpna3
    • Qip2
    • SRP1
    • SRP1-gamma
    • SRP1gamma
    • SRP4
    see all
  • FunctionFunctions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. Recognizes NLSs of influenza A virus nucleoprotein probably through ARM repeats 7-9.
  • Tissue specificityUbiquitous. Highest levels in heart and skeletal muscle.
  • Sequence similaritiesBelongs to the importin alpha family.
    Contains 10 ARM repeats.
    Contains 1 IBB domain.
  • DomainConsists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
    The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
    The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt

Recombinant Human KPNA3 protein images

  • 12.5% SDS-PAGE showing ab114747 at approximately 36.63kDa.
    Stained with Coomassie Blue.

References for Recombinant Human KPNA3 protein (ab114747)

ab114747 has not yet been referenced specifically in any publications.

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