Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MQEEDLPRPS ISAEPGTVIP LGSHVTFVCR GPVGVQTFRL ERESRSTYND TEDVSQASPS ESEARFRIDS VSEGNAGPYR CIYYKPPKWS EQSDYLELLV KETSG
    • Amino acids
      22 to 125

Associated products

Specifications

Our Abpromise guarantee covers the use of ab51219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.
    The extracellular domain of LAIR 1( 22-125aa) was overexpressed in E.coli, and purified by using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50mM Sodium chloride, 20mM Tris HCl, 1mM EDTA, pH 7.5

General Info

  • Alternative names
    • 5133400O11Rik
    • CD305
    • D7Bwg0421e
    • hLAIR1
    • LAIR 1
    • LAIR-1
    • LAIR1
    • LAIR1 protein
    • LAIR1_HUMAN
    • Leukocyte associated Ig like receptor 1
    • Leukocyte associated immunoglobulin like receptor 1
    • Leukocyte-associated immunoglobulin-like receptor 1
    see all
  • Function
    Functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ T-cells, downregulating IL2 and IFNG production while inducing secretion of transforming growth factor beta. Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors towards dendritic cells.
  • Tissue specificity
    Expressed on the majority of peripheral mononuclear cells, including natural killer (NK) cells, T-cells, B-cells, monocytes, and dendritic cells. Highly expressed in naive T-cells and B-cells but no expression on germinal center B-cells. Abnormally low expression in naive B-cells from HIV-1 infected patients. Very low expression in NK cells from a patient with chronic active Epstein-Barr virus infection.
  • Sequence similarities
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • Developmental stage
    Complete loss of expression when naive B-cells proliferates and differentiates into Ig-producing plasma cells under in vitro stimulation.
  • Domain
    ITIM (immunoreceptor tyrosine-based inhibitor motif) motif is a cytoplasmic motif present in 2 copies in the intracellular part of LAIR1. When phosphorylated, ITIM motif can bind the SH2 domain of several SH2-containing phosphatases, leading to down-regulation of cell activation.
  • Post-translational
    modifications
    Phosphorylation at Tyr-251 and Tyr-281 activates it. May be phosphorylated by LCK.
    N-glycosylated.
  • Cellular localization
    Cell membrane.
  • Information by UniProt

Images

  • ab51219 in 14% SDS-PAGE

References

ab51219 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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