Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      16 kDa
    • Amino acids
      1 to 120
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab103506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 95 % SDS-PAGE.

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0

General Info

  • Alternative names
    • ATG8F
    • Autophagy-related protein LC3 B
    • Autophagy-related ubiquitin-like modifier LC3 B
    • LC3B
    • LC3II
    • MAP1 light chain 3 like protein 2
    • MAP1 light chain 3-like protein 2
    • MAP1A/1BLC3
    • MAP1A/MAP1B LC3 B
    • MAP1A/MAP1B light chain 3 B
    • MAP1ALC3
    • Map1lc3b
    • MAP1LC3B a
    • Microtubule associated protein 1 light chain 3 beta
    • Microtubule-associated protein 1 light chain 3 beta
    • Microtubule-associated proteins 1A/1B light chain 3B
    • MLP3B_HUMAN
    see all
  • Function
    Probably involved in formation of autophagosomal vacuoles (autophagosomes).
  • Tissue specificity
    Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.
  • Sequence similarities
    Belongs to the MAP1 LC3 family.
  • Post-translational
    modifications
    The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II.
  • Cellular localization
    Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
  • Information by UniProt

Images

  • 15% SDS PAGE analysis of ab103506 (3 µg).

References

ab103506 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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