Recombinant human LDHA protein (His tag) (ab93699)

Overview

  • Product name
    Recombinant human LDHA protein (His tag)
  • Protein length
    Full length protein

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MGSSHHHHHH SSGLVPRGSH MATLKDQLIY NLLKEEQTPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG EMMDLQHGSL FLRTPKIVSG KDYNVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPN CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPV WSGMNVAGVS LKTLHPDLGT DKDKEQWKEV HKQVVESAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPVSTMIKGL YGIKDDVFLS VPCILGQNGI SDLVKVTLTS EEEARLKKSA DTLWGIQKEL QF

Specifications

Our Abpromise guarantee covers the use of ab93699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Specific activity is > 300 units/mg. in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37°C.

    Activity Assay

    1. Prepare a 1.5 ml reaction mix into a suitable container, adjust to pH 7.5 at 37°C and pre-chill on ice before use: The final concentrations are 100mM Sodium phosphate, 0.12 mM beta-nicotinamide adenine dinucleotide, reduced form, 2.3mM Pyruvate, 0.033% (w/v) bovine serum albumin.
    2. Equilibrate to 37°C and monitor at A340nm until the value is constant using a spectrophotometer.
    3. Add 50ul of recombinant LDHA protein in various concentrations (0.1ug, 0.5ug) in assay buffer.
    4. Mix by inversion and record the increase at A340nm for 5 minutes.
  • Applications

    Functional Studies

    SDS-PAGE

    Western blot

  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 95 % SDS-PAGE.
    ab93699 is purified by using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 20% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Cell proliferation-inducing gene 19 protein
    • GSD11
    • L lactate dehydrogenase A chain
    • L-lactate dehydrogenase A chain
    • l7R2
    • Lactate dehydrogenase 1, A chain
    • Lactate dehydrogenase A
    • Lactate dehydrogenase A4
    • Lactate dehydrogenase M
    • LDH A
    • LDH M
    • LDH muscle subunit
    • LDH muscle subunit; M LDH
    • LDH-A
    • LDH-M
    • LDH1
    • ldha
    • LDHA_HUMAN
    • LDHM
    • OTTMUSP00000017774
    • PIG19
    • Proliferation-inducing gene 19
    • Renal carcinoma antigen NY-REN-59
    see all
  • Pathway
    Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
  • Involvement in disease
    Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
  • Sequence similarities
    Belongs to the LDH/MDH superfamily. LDH family.
  • Post-translational
    modifications
    ISGylated.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

Images

  • 15% SDS-PAGE showing ab93699 at approximately 38.8kDa (3µg).

References

ab93699 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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