The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityDetermined by its ability to chemoattract total human monocytes using a concentration range of 10-100 ng/ml corresponding to a Specific Activity of 1,000-10,000IU/mg.
% SDS-PAGE. ab116400 purified by proprietary chromatographic techniques.
Additional notesLyophilized ab116400 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, it should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionIt is recommended to reconstitute the lyophilized protein in sterile ultra pure water not less than 100µg/ml, which can then be further diluted into other aqueous solutions.
C-C motif chemokine 16
Chemokine (C-C motif) ligand 16
IL10 inducible chemokine
Liver CC chemokine 1 precursor
Liver expressed chemokine
Lymphocyte and monocyte chemoattractant
New CC chemokine 4
Small inducible cytokine A16 precursor
Small inducible cytokine subfamily A (Cys Cys) member 16
Small-inducible cytokine A16
FunctionShows chemotactic activity for lymphocytes and monocytes but not neutrophils. Also shows potent myelosuppressive activity, suppresses proliferation of myeloid progenitor cells. Recombinant SCYA16 shows chemotactic activity for monocytes and THP-1 monocytes, but not for resting lymphocytes and neutrophils. Induces a calcium flux in THP-1 cells that were desensitized by prior expression to RANTES.
Tissue specificityMainly expressed in liver, also found in spleen and thymus. Highly expressed in LPS- and IFN-gamma-activated monocytes, weakly in some lymphocytes, including natural killer cells, gamma-delta T-cells, and some T-cell clones.
Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.