Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP41159
    • SpeciesHuman
    • SequenceAVPIQKVQDD TKTLIKTIVT RINDISHTQS VSSKQKVTGL DFIPGLHPIL TLSKMDQTLA VYQQILTSMP SRNVIQISND LENLRDLLHV LAFSKSCHLP WASGLETLDS LGGVLEASGY STEVVALSRL QGSLQDMLWQ LDLSPGC
    • Amino acids21 to 167

Specifications

Our Abpromise guarantee covers the use of ab13987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking

    Western blot

    ELISA

  • Purity> 95 % SDS-PAGE.

  • FormLyophilised
  • Additional notesStore lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituent: 0.04% Sodium bicarbonate

  • ReconstitutionAdd 0.2 ml of deionized water and let the lyophilized pellet dissolve completely. Once the protein is solubilized, pH can be readjusted to the physiological range. Store lyophilized protein at -20°C

General Info

  • Alternative names
    • FLJ94114
    • LEP
    • LEP_HUMAN
    • LEPD
    • Leptin
    • Leptin (murine obesity homolog)
    • Leptin (obesity homolog, mouse)
    • Leptin Murine Obesity Homolog
    • Leptin Precursor Obesity Factor
    • OB
    • Obese protein
    • Obese, mouse, homolog of
    • Obesity
    • Obesity factor
    • Obesity homolog mouse
    • Obesity Murine Homolog Leptin
    • OBS
    • OTTHUMP00000212285
    see all
  • FunctionMay function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
  • Involvement in diseaseDefects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • Sequence similaritiesBelongs to the leptin family.
  • Cellular localizationSecreted.
  • Information by UniProt

References for Recombinant Human Leptin protein (ab13987)

This product has been referenced in:
  • Mebis L  et al. Contribution of nutritional deficit to the pathogenesis of the nonthyroidal illness syndrome in critical illness: a rabbit model study. Endocrinology 153:973-84 (2012). Blocking . Read more (PubMed: 22166982) »

See 1 Publication for this product

Product Wall

Thank you for passing some information to me.
I understand that ab3583 has been used previously at 1:4000.
Have you had a chance to test this antibody at dilutions of 1/500 or 1/1000 (for 1 hr at room temperature or overnight at 4oC?
Wou...

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Thank you for getting back to me and for specifying the sample types.
As you have kindly explained to me in your previous e-mail, the primary and the secondary antibodies worked with the positive control but the signal was very weak using lysates ...

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Thank you for confirming the order dfetails.
Would you be so kind to clarify what samples you have been using:
- species,
- cell or tissue types,
- lysate (total cell lysates, or cytoplasmic fraction etc)
I look forward to hear...

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Thank you for your prompt answers. Your co-operation in this matter is highly appreciated.
I understand that you have already carried out some optimization steps and it seems that the primary and the secondary antibodies worked on positive control...

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Thank you for enquiry and for taking the time to provide some useful details of the experiments. As I understand three of our products (ab3583, ab13987 and ab97051) have been used in these studies.
Though you have kindly provided some details, it ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"