The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activitySpecific activity is not less than 1 x 108 units/mg. Biological activity: measured by the ability to stimulate the differentiation of the M1 mouse myeloid leukemic cell line.
50 units is defined as the amount of mouse LIF required to induce differentiation in 50% of the M1 colonies in 1 mL agar cultures.
% SDS-PAGE. Purity: >95% by SDS-PAGE and HPLC
Endotoxin: <0.1 ng/µg LIF
Additional notesDilute the contents of the vial using 0.2 µm-filtered PBS containing 1% BSA or 0.02% TWEEN 20 to a final concentration of not less than 1.0 µg/ml.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Cholinergic Differentiation Factor
Differentiation inducing factor
differentiation inhibitory activity
Differentiation stimulating factor
Hepatocyte stimulating factor III
Human interleukin in DA cells
Leukemia inhibitory factor
Melanoma derived LPL inhibitor
Melanoma-derived LPL inhibitor
FunctionLIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
Sequence similaritiesBelongs to the LIF/OSM family.