Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman

Associated products

Specifications

Our Abpromise guarantee covers the use of ab57665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySpecific activity is not less than 1 x 108 units/mg.
    Biological activity: measured by the ability to stimulate the differentiation of the M1 mouse myeloid leukemic cell line. 50 units is defined as the amount of mouse LIF required to induce differentiation in 50% of the M1 colonies in 1 mL agar cultures.
  • Applications

    SDS-PAGE

    Functional Studies

    Western blot

  • Purity> 95 % SDS-PAGE.
    Purity: >95% by SDS-PAGE and HPLC Endotoxin: <0.1 ng/µg LIF
  • FormLiquid
  • Additional notesDilute the contents of the vial using 0.2 µm-filtered PBS containing 1% BSA or 0.02% TWEEN 20 to a final concentration of not less than 1.0 µg/ml.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C.

    Preservative: None
    Constituents: 0.02% Tween 20, PBS, pH 7.4

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • CDF
    • Cholinergic Differentiation Factor
    • D factor
    • DIA
    • Differentiation inducing factor
    • differentiation inhibitory activity
    • Differentiation stimulating factor
    • Differentiation-stimulating factor
    • Emfilermin
    • Hepatocyte stimulating factor III
    • HILDA
    • Human interleukin in DA cells
    • Leukemia inhibitory factor
    • LIF
    • LIF_HUMAN
    • Melanoma derived LPL inhibitor
    • Melanoma-derived LPL inhibitor
    • MLPLI
    see all
  • FunctionLIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
  • Sequence similaritiesBelongs to the LIF/OSM family.
  • Cellular localizationSecreted.
  • Information by UniProt

Recombinant human LIF protein images

  • Anti-LIF antibody (ab104705) at 1 µg/ml + Recombinant human LIF protein (ab57665) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 8 minutes

References for Recombinant human LIF protein (ab57665)

ab57665 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"