The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityThe ED50 determined by the M1 cell differentiation assay is <
0.01 ng/ml, corresponding to a specific activity of 100 MIU/mg.
% SDS-PAGE. Purity is >95% by SDS-PAGE and HPLC analyses.
Additional notesEndotoxin level is <0.1 ng per µg.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 1X PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionCentrifuge the vial prior to opening. Reconstitute in water to a
concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week, or as working aliquots at –20°C for future use. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Cholinergic Differentiation Factor
Differentiation inducing factor
differentiation inhibitory activity
Differentiation stimulating factor
Hepatocyte stimulating factor III
Human interleukin in DA cells
Leukemia inhibitory factor
Melanoma derived LPL inhibitor
Melanoma-derived LPL inhibitor
FunctionLIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
Sequence similaritiesBelongs to the LIF/OSM family.