Recombinant Human Lipoamide Dehydrogenase protein (ab116690)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP09622
    • SpeciesHuman
    • SequenceMRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMADQ PIDADVTVIG SGPGGYVAAI KAAQLGFKTV CIEKNETLGG TCLNVGCIPS KALLNNSHYY HMAHGKDFAS RGIEMSEVRL NLDKMMEQKS TAVKALTGGI AHLFKQNKVV HVNGYGKITG KNQVTATKAD GGTQVIDTKN ILIATGSEVT PFPGITIDED TIVSSTGALS LKKVPEKMVV IGAGVIGVEL GSVWQRLGAD VTAVEFLGHV GGVGIDMEIS KNFQRILQKQ GFKFKLNTKV TGATKKSDGK IDVSIEAASG GKAEVITCDV LLVCIGRRPF TKNLGLEELG IELDPRGRIP VNTRFQTKIP NIYAIGDVVA GPMLAHKAED EGIICVEGMA GGAVHIDYNC VPSVIYTHPE VAWVGKSEEQ LKEEGIEYKV GKFPFAANSR AKTNADTDGM VKILGQKSTD RVLGAHILGP GAGEMVNEAA LALEYGASCE DIARVCHAHP TLSEAFREAN LAASFGKSIN MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMADQ PIDADVTVIG SGPGGYVAAI KAAQLGFKTV CIEKNETLGG TCLNVGCIPS KALLNNSHYY HMAHGKDFAS RGIEMSEVRL NLDKMMEQKS TAVKALTGGI AHLFKQNKVV HVNGYGKITG KNQVTATKAD GGTQVIDTKN ILIATGSEVT PFPGITIDED TIVSSTGALS LKKVPEKMVV IGAGVIGVEL GSVWQRLGAD VTAVEFLGHV GGVGIDMEIS KNFQRILQKQ GFKFKLNTKV TGATKKSDGK IDVSIEAASG GKAEVITCDV LLVCIGRRPF TKNLGLEELG IELDPRGRIP VNTRFQTKIP NIYAIGDVVA GPMLAHKAED EGIICVEGMA GGAVHIDYNC VPSVIYTHPE VAWVGKSEEQ LKEEGIEYKV GKFPFAANSR AKTNADTDGM VKILGQKSTD RVLGAHILGP GAGEMVNEAA LALEYGASCE DIARVCHAHP TLSEAFREAN LAASFGKSIN F
    • Molecular weight54 kDa including tags
    • Amino acids36 to 509
    • TagsHis tag N-Terminus
    • Additional sequence informationDLD Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 511 amino acids (36-509 a.a.). DLD is fused to a 37 amino acid His Tag at N-terminus.

Specifications

Our Abpromise guarantee covers the use of ab116690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    ab116690 is purified by proprietary chromatographic techniques.
  • FormLiquid
  • Additional notesAlthough stable at 4°C for 30 days, ab116690 should be stored desiccated below -20°C for periods greater than 30 days. Please avoid freeze-thaw cycles.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Please see notes section.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 0.02% DTT, 0.58% Sodium chloride, 10% Glycerol

General Info

  • Alternative names
    • Dehydrogenase complex, E3 component
    • Diaphorase
    • Dihydrolipoamide dehydrogenase
    • Dihydrolipoyl dehydrogenase
    • Dihydrolipoyl dehydrogenase mitochondrial
    • dld
    • DLDD
    • DLDH
    • DLDH_HUMAN
    • E3
    • E3 branched chain aplha-keto acid
    • E3 component of pyruvate dehydrogenase
    • E3 component of pyruvate dehydrogenase complex 2 oxo glutarate complex branched chain keto acid dehydrogenase complex
    • GCSL
    • Glycine cleavage system L protein
    • Glycine cleavage system protein L
    • LAD
    • lipoamide dehydrogenase
    • Lipoamide reductase
    • Lipoyl dehydrogenase
    • mitochondrial
    • OTTHUMP00000206744
    • OTTHUMP00000206746
    • OTTHUMP00000206748
    • OTTHUMP00000206749
    • PHE 3
    • PHE3
    • Pyruvate dehydrogenase component E3
    see all
  • FunctionLipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.
  • Involvement in diseaseNote=Defects in DLD are involved in the development of congenital infantile lactic acidosis.
    Defects in DLD are a cause of maple syrup urine disease (MSUD) [MIM:248600]. MSUD is characterized by mental and physical retardation, feeding problems and a maple syrup odor to the urine. The keto acids of the branched-chain amino acids are present in the urine, resulting from a block in oxidative decarboxylation.
  • Sequence similaritiesBelongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
  • Post-translational
    modifications
    Tyrosine phosphorylated.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt

References for Recombinant Human Lipoamide Dehydrogenase protein (ab116690)

ab116690 has not yet been referenced specifically in any publications.

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