Recombinant Human Lipoprotein lipase (ab115500)
Key features and details
- Expression system: Escherichia coli
- Purity: > 75% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: WB
Description
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Product name
Recombinant Human Lipoprotein lipase
See all Lipoprotein lipase proteins and peptides -
Purity
> 75 % SDS-PAGE.
ab115500 is purified using Ni-NTA chromatography and filtered (0.4 µm) before being lyophilised. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MKHHHHHHASADQRRDFIDIESKFALRTPEDTAEDTCHLIPGVAESVATC HFNHSSKTFMVIHGWTVTGMYESWVPKLVAALYKREPDSNVIVVDWLSRA QEHYPVSAGYTKLVGQDVARFINWMEEEFNYPLDNVHLLGYSLGAHAAGI AGSLTNKKVNRITGLDPAGPNFEYAEAPSRLSPDDADFVDVLHTFTRGSP GRSIGIQKPVGHVDIYPNGGTFQPGCNIGEAIRVIAERGLGDVDQLVKCS HERSIHLFIDSLLNEENPSKAYRCSSKEAFEKGLCLSCRKNRCNNLGYEI SKVRAKRSSKMYLKTRSQMPYKVFHYQVKIHFSGTESETHTNQAFEISLY GTVAESENIPFTLPEVSTNKTYSFLIYTEVDIGELLMLKLKWKSDSYFSW SDWWSSPGFAIQKIRVKAGETQKKVIFCSREKVSHLQKGKAPAVFVKCHD KSLNKKSG -
Predicted molecular weight
52 kDa including tags -
Amino acids
28 to 475 -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab115500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C.
Constituents: 0.3% Acetic acid, 0.4% Sodium acetate
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ReconstitutionTo reconstitute, add 0.1M Acetate buffer pH4. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a week. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
General Info
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Alternative names
- EC 3.1.1
- EC 3.1.1.34
- HDLCQ11
see all -
Function
The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium. -
Involvement in disease
Defects in LPL are the cause of lipoprotein lipase deficiency (LPL deficiency) [MIM:238600]; also known as familial chylomicronemia or hyperlipoproteinemia type I. LPL deficiency chylomicronemia is a recessive disorder usually manifesting in childhood. On a normal diet, patients often present with abdominal pain, hepatosplenomegaly, lipemia retinalis, eruptive xanthomata, and massive hypertriglyceridemia, sometimes complicated with acute pancreatitis. -
Sequence similarities
Belongs to the AB hydrolase superfamily. Lipase family.
Contains 1 PLAT domain. -
Post-translational
modificationsTyrosine nitration after lipopolysaccharide (LPS) challenge down-regulates the lipase activity. -
Cellular localization
Cell membrane. Secreted. Locates to the plasma membrane of microvilli of hepatocytes with triacyl-glycerol-rich lipoproteins (TRL). Some of the bound LPL is then internalized and located inside non-coated endocytic vesicles. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab115500 has not yet been referenced specifically in any publications.