Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
      Human
    • Tags
      proprietary tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab60306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific activity: 235nmol/min/mg.
  • Applications

    Functional Studies

    SDS-PAGE

    Western blot

  • Form
    Liquid
  • Additional notes

    ab204883 (CDK1 peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • AA407514
    • EC 2.7.10.2
    • FLJ26625
    • Hck 2
    • JTK 8
    • JTK8
    • Lck/Yes related novel protein tyrosine kinase
    • LYN
    • LYN proto oncogene, Src family tyrosine kinase
    • LYN_HUMAN
    • ONCOGENE LYN
    • p53Lyn
    • p56Lyn
    • Tyrosine protein kinase LYN
    • Tyrosine-protein kinase Lyn
    • V yes 1 Yamaguchi sarcoma viral related oncogene homolog
    • Yamaguchi sarcoma viral (v yes 1) related oncogene homolog
    see all
  • Function
    Down regulates expression of stem cell growth factor receptor (KIT). Acts as an effector of EpoR (erythropoietin receptor) in controlling KIT expression and may play a central role in erythroid differentiation during the switch between proliferation and maturation (By similarity). Acts as a positive regulator of cell movement while negatively regulating adhesion to stromal cells by inhibiting the ICAM-1-binding activity of beta-2 integrins. Acts as the mediator that relays suppressing signals from the chemokine receptor CXCR4 to beta-2 integrin LFA-1 in hematopoietic precursors. Involved in induction of stress-activated protein kinase (SAPK), but not ERK or p38 MAPK, in response to genotoxic agents. Induces SAPK by a MKK7- and MEKK1-dependent mechanism. The LYN -> MEKK1 -> MKK7 -> SAPK pathway is functional in the induction of apoptosis by genotoxic agents.
  • Tissue specificity
    Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Domain
    The protein kinase domain plays an important role in its localization in the cell membrane.
  • Post-translational
    modifications
    Ubiquitinated. Ubiquitination is SH3-dependent.
  • Cellular localization
    Cell membrane. Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Golgi apparatus. Accumulates in the nucleus by inhibition of CRM1-mediated nuclear export. Nuclear accumulation is increased by inhibition of its kinase activity. The trafficking from the Golgi apparatus to the plasma membrane occurs in a kinase domain-dependent but kinase activity independent manner and is mediated by exocytic vesicular transport.
  • Information by UniProt

Images

  • Sample Kinase assay plot.
  • ab60306 on SDS-PAGE.

References

ab60306 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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