Overview

  • Product nameRecombinant Human MAGEA3 protein
  • Protein lengthFull length protein

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP43357
    • SpeciesHuman
    • SequenceMGSSHHHHHHSSGLVPRGSHMGSMPLEQRSQHCKPEEGLEARGEALGLVG AQAPATEEQEAASSSSTLVEVTLGEVPAAESPDPPQSPQGASSLPTTMNY PLWSQSYEDSSNQEEEGPSTFPDLESEFQAALSRKVAELVHFLLLKYRAR EPVTKAEMLGSVVGNWQYFFPVIFSKASSSLQLVFGIELMEVDPIGHLYI FATCLGLSYDGLLGDNQIMPKAGLLIIVLAIIAREGDCAPEEKIWEELSV LEVFEGREDSILGDPKKLLTQHFVQENYLEYRQVPGSDPACYEFLWGPRA LVETSYVKVLHHMVKISGGPHISYPPLHEWVLREGEE
    • Molecular weight37 kDa including tags
    • Amino acids1 to 314
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab99958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity> 90 % SDS-PAGE.
    ab99958 is purified using conventional chromatography techniques.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 100mM Sodium chloride, 1mM DTT, pH 8.0

General Info

  • Alternative names
    • Antigen MZ2 D
    • Antigen MZ2-D
    • Cancer/testis antigen 1.3
    • Cancer/testis antigen family 1 member 3
    • CT1.3
    • HIP8
    • HYPD
    • MAGA3_HUMAN
    • MAGE 3 antigen
    • MAGE-3 antigen
    • MAGE3
    • MAGEA3
    • MAGEA6
    • Melanoma antigen family A 3
    • Melanoma associated antigen 3
    • Melanoma-associated antigen 3
    • MGC1461
    see all
  • FunctionProposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.
  • Tissue specificityExpressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta. Never expressed in kidney tumors, Leukemias and lymphomas.
  • Sequence similaritiesContains 1 MAGE domain.
  • Information by UniProt

Recombinant Human MAGEA3 protein images

  • 15% SDS-PAGE showing ab99958 (3µg).

References for Recombinant Human MAGEA3 protein (ab99958)

ab99958 has not yet been referenced specifically in any publications.

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