Recombinant Human MAP1LC3B2 protein (ab106858)

Overview

  • Product name
    Recombinant Human MAP1LC3B2 protein
  • Protein length
    Full length protein

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHHSSGLVPRGSHMPSEKTFKQRRTFEQRVEDVRLIREQHPTK IPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFF LLVNGHSMVSVSTPISEVYESEKDEDGFLYMVCASQETFG
    • Molecular weight
      16 kDa including tags
    • Amino acids
      1 to 120
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab106858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 95 % SDS-PAGE.
    ab106858 is purified using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0

General Info

  • Alternative names
    • ATG8G
    • MAP1LC3B2
    • Microtubule associated protein 1 light chain 3 beta 2
    • Microtubule associated proteins 1A/1B light chain 3B like protein
    • Microtubule-associated proteins 1A/1B light chain 3 beta 2
    • Microtubule-associated proteins 1A/1B light chain 3B-like
    • MP3B2_HUMAN
    see all
  • Function
    Probably involved in formation of autophagosomal vacuoles (autophagosomes).
  • Sequence similarities
    Belongs to the MAP1 LC3 family.
  • Post-translational
    modifications
    The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I (By similarity). This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II.
  • Cellular localization
    Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of 3µg ab106858.

References

ab106858 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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