Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
      Human
    • Amino acids
      30 to 397

Specifications

Our Abpromise guarantee covers the use of ab55700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific activity: 1012 nmol/min/mg
  • Applications

    SDS-PAGE

    Functional Studies

  • Form
    Liquid
  • Additional notes

    ab43623 (Human ERK1 full length protein) and ab43614 (Human Myelin Basic Protein full length protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • AURA2
    • c COT
    • Cancer Osaka thyroid oncogene
    • CCOT
    • COT
    • COT proto oncogene serine/threonine protein kinase
    • EST
    • ESTF
    • Ewing sarcoma transformant
    • FLJ10486
    • M3K8_HUMAN
    • MAP3K 8
    • MAP3K8
    • MEKK8
    • Mitogen activated protein kinase kinase kinase 8
    • Mitogen-activated protein kinase kinase kinase 8
    • Proto oncogene cCot
    • Proto-oncogene c-Cot
    • Serine/threonine protein kinase cot
    • Serine/threonine-protein kinase cot
    • TPL 2
    • TPL-2
    • TPL2
    • Tumor progression locus 2
    see all
  • Function
    Required for TLR4 activation of the MEK/ERK pathway. Able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. Plays a role in the cell cycle. The longer form has some transforming activity, although it is much weaker than the activated cot oncoprotein.
  • Tissue specificity
    Expressed in several normal tissues and human tumor-derived cell lines.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Developmental stage
    Isoform 1 is activated specifically during the S and G2/M phases of the cell cycle.
  • Post-translational
    modifications
    Autophosphorylated. Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr residues.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

Images

  • Kinase activity plot using ab55700. Specific activity: 1012 nmol/min/mg.
  • SDS-PAGE analysis of ab55700, with molecular weight markers. Approximate molecular weight: 70kDa

References

ab55700 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab55700.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up