Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • SpeciesHuman
    • Amino acids30 to 397
    • TagsGST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab84728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Sodium chloride, 50mM Tris HCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

General Info

  • Alternative names
    • c COT
    • Cancer Osaka thyroid oncogene
    • CCOT
    • COT
    • COT proto oncogene serine/threonine protein kinase
    • EST
    • ESTF
    • Ewing sarcoma transformant
    • FLJ10486
    • M3K8_HUMAN
    • MAP3K 8
    • MAP3K8
    • MEKK8
    • Mitogen activated protein kinase kinase kinase 8
    • Mitogen-activated protein kinase kinase kinase 8
    • Proto oncogene cCot
    • Proto-oncogene c-Cot
    • Serine/threonine protein kinase cot
    • Serine/threonine-protein kinase cot
    • TPL 2
    • TPL-2
    • TPL2
    • Tumor progression locus 2
    see all
  • FunctionRequired for TLR4 activation of the MEK/ERK pathway. Able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. Plays a role in the cell cycle. The longer form has some transforming activity, although it is much weaker than the activated cot oncoprotein.
  • Tissue specificityExpressed in several normal tissues and human tumor-derived cell lines.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Developmental stageIsoform 1 is activated specifically during the S and G2/M phases of the cell cycle.
  • Post-translational
    modifications
    Autophosphorylated. Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr residues.
  • Cellular localizationCytoplasm.
  • Information by UniProt

Recombinant Human MAP3K8 protein images

  • ab84728 by SDS-Page

References for Recombinant Human MAP3K8 protein (ab84728)

ab84728 has not yet been referenced specifically in any publications.

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